RestStart PCRStart SphI NarI L G S S P V V G Z G R Z A P P P Q G M V H A R R W R P TTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCC ^10 ^20 ^30 ^40 ^50 ^60 ^70 AATCCTTCGTCGGGTCATCATCCAACTCCGGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTACCGCGGG -------------------------------------------------------------------------------- MboI HpaII NdeII MspI MboII T V P R P R G L P P Y P R R N K R S Z A R S G E P D L AACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATC ^90 ^100 ^110 ^120 ^130 ^140 ^150 TTGTCAGGGGGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACCGCTCGGGCTAG -------------------------------------------------------------------------------- HpaII NaeI MspI HpaII HpaII NarI NarI MspI MspI P H R Z C R R Y R R Q Q P H L W R R Z C R P R C V R TTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGG ^170 ^180 ^190 ^200 ^210 ^220 ^230 AAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACACCGCGGCCACTACGGCCGGTGCTACGCAGGCC -------------------------------------------------------------------------------- 5' taatacgactcactatagg 3' pet5prime19mer BglII MboI NdeII TaqI MboI MboI NdeII NdeII TaqI VspI HinfI XbaI R R G S R S R S R E I N T T H Y R G I V S G Z Q F P S CGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC ^250 ^260 ^270 ^280 ^290 ^300 ^310 GCATCTCCTAGCTCTAGAGCTAGGGCGCTTTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAG -------------------------------------------------------------------------------- NdeI DraI R N N F V Z L Z E G D I H M E Q R I T L K D Y A M R F TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAcatATGGAACAACGCATAACTTTAAAAGATTATGCAATGCGCT ^330 ^340 ^350 ^360 ^370 ^380 ^390 ATCTTTATTAAAACAAATTGAAATTCTTCCTCTATATgtaTACCTTGTTGCGTATTGAAATTTTCTAATACGTTACGCGA -------------------------------------------------------------------------------- BglII MboI NaeI NdeII MboI HpaII AluI NdeII MspI SspI G Q T K T A K D L G V Y Q S A I N K A I H A G R K I TTGGCCAAACCAAGACAGCTAAAGATCTCGGCGTGTATCAAAGCGCGATCAACAAGGCCATCCATGCCGGCCGAAAAATA ^410 ^420 ^430 ^440 ^450 ^460 ^470 AACCGGTTTGGTTCTGTCGATTTCTAGAGCCGCACATAGTTTCGCGCTAGTTGTTCCGGTAGGTACGGCCGGCTTTTTAT -------------------------------------------------------------------------------- BamHI MboI AvaI NdeII MboII F L T I N A D G S V Y A E E V K P F P S N K K T T A A TTTTTGACTATAAACGCTGATGGATCCGTATACGCGGAAGAGGTAAAGCCCTTCCCGAGTAACAAAAAAACAACAGCGGC ^490 ^500 ^510 ^520 ^530 ^540 ^550 AAAAACTGATATTTGCGACTACCTAGGCATATGCGCCTTCTCCATTTCGGGAAGGGCTCATTGTTTTTTTGTTGTCGCCG -------------------------------------------------------------------------------- HpaII KpnI MspI RsaI HpaII MspI DraI G T G G S G G M E Q R I T L K D Y A M R F G Q T K T A CGGTACCGGTGGCTCTGGCGGCATGGAACAACGCATAACTTTAAAAGATTATGCAATGCGCTTTGGCCAAACCAAGACAG ^570 ^580 ^590 ^600 ^610 ^620 ^630 GCCATGGCCACCGAGACCGCCGTACCTTGTTGCGTATTGAAATTTTCTAATACGTTACGCGAAACCGGTTTGGTTCTGTC -------------------------------------------------------------------------------- BglII MboI NaeI NdeII MboI HpaII AluI NdeII MspI SspI K D L G V Y Q S A I N K A I H A G R K I F L T I N A CTAAAGATCTCGGCGTGTATCAAAGCGCGATCAACAAGGCCATCCATGCCGGCCGAAAAATATTTTTGACTATAAACGCT ^650 ^660 ^670 ^680 ^690 ^700 ^710 GATTTCTAGAGCCGCACATAGTTTCGCGCTAGTTGTTCCGGTAGGTACGGCCGGCTTTTTATAAAAACTGATATTTGCGA -------------------------------------------------------------------------------- BamHI TaqI MboI AvaI AvaI NdeII MboII XhoI D G S V Y A E E V K P F P S N K K T T A Z I L E H H H GATGGATCCGTATACGCGGAAGAGGTAAAGCCCTTCCCGAGTAACAAAAAAACAACAGCATAAataCTCGAGcaccacca ^730 ^740 ^750 ^760 ^770 ^780 ^790 CTACCTAGGCATATGCGCCTTCTCCATTTCGGGAAGGGCTCATTGTTTTTTTGTTGTCGTATTtatGAGCTCgtggtggt -------------------------------------------------------------------------------- 3' ggcgactcgttattgatc HpaII MspI MboI NdeII AluI H H H Z D P A A N K A R K E A E L A A A T A E Q Z L A cCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG ^810 ^820 ^830 ^840 ^850 ^860 ^870 gGTGGTGGTGACTCTAGGCCGACGATTGTTTCGGGCTTTCCTTCGACTCAACCGACGACGGTGGCGACTCGTTATTGATC -------------------------------------------------------------------------------- RestEnd PCREnd 5' pet3prime18mer HpaII MspI AccIII Z P L G A S K R V L R G F L L K G G T I S G CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT ^890 ^900 ^910 ^920 ^930 ^940 GTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAAAACGACTTTCCTCCTTGATATAGGCCTA ---------------------------------------------------------------------
| Restriction Summary |
| AatII gacgt|c: none. |
| AccIII t|ccgga: 944. |
| AluI ag|ct: 419, 641, 845. |
| ApaI gggcc|c: none. |
| AvaI c|ycgrg: 535, 757, 788. |
| BamHI g|gatcc: 503, 725. |
| BclI t|gatca: none. |
| BglII a|gatct: 254, 424, 646. |
| BssHII g|cgcgc: none. |
| ClaI at|cgat: none. |
| DraI ttt|aaa: 381, 603. |
| EcoRI g|aattc: none. |
| EcoRV gat|atc: none. |
| HincII gty|rac: none. |
| HindIII a|agctt: none. |
| HinfI g|antc: 280. |
| HpaI gtt|aac: none. |
| HpaII c|cgg: 91, 214, 223, 238, 468, 561, 567, 690, 818, 945. |
| KpnI ggtac|c: 567. |
| MboI |gatc: 157, 248, 254, 260, 424, 447, 503, 646, 669, 725, 814. |
| MboII gaagannnnnnnn| MboII n|nnnnnnntcttc: 152, 530, 752. |
| MluI a|cgcgt: none. |
| MspI c|cgg: 91, 214, 223, 238, 468, 561, 567, 690, 818, 945. |
| NaeI gcc|ggc: 224, 469, 691. |
| NarI gg|cgcc: 76, 190, 211. |
| NcoI c|catgg: none. |
| NdeI ca|tatg: 360. |
| NdeII |gatc: 157, 248, 254, 260, 424, 447, 503, 646, 669, 725, 814. |
| NheI g|ctagc: none. |
| NotI gc|ggccgc: none. |
| NruI tcg|cga: none. |
| PstI ctgca|g: none. |
| PvuI cgat|cg: none. |
| PvuII cag|ctg: none. |
| RsaI gt|ac: 565. |
| SacI gagct|c: none. |
| SacII ccgc|gg: none. |
| SalI g|tcgac: none. |
| ScaI agt|act: none. |
| SmaI ccc|ggg: none. |
| SpeI a|ctagt: none. |
| SphI gcatg|c: 65. |
| SspI aat|att: 480, 702. |
| StuI agg|cct: none. |
| TaqI t|cga: 251, 259, 789. |
| VspI at|taat: 273. |
| XbaI t|ctaga: 320. |
| XhoI c|tcgag: 788. |
| XmaI c|ccggg: none. |
| Primer Summary |
| pet3prime18mer ctagttattgctcagcgg: -881. |
| pet5prime19mer taatacgactcactatagg: 273. |