CTAGTTATTGCTCAGCGG pET-lacZ726a,
CAGTCGACGAATTCTTTTGTTTAACTTTAAGAAGGAGA pET-SD726b,
CAGTCGACGAATTCCAGGAAACAGAATGGTTGCAT CroSD726c,
CCTCGAGTGTAAAACGACGGCCAGTG, CroCterm726d,
CGTCATCGCCATTAATTCACTGAT pAH153RIGHT,
GAACGCTCTCCTGAGTAGGACAAA pAH153 LEFT PRIMER,
CAATTCCGACGTCTAAGAAACCAT GFP-LEFT PRIMER,
AAAATTGGGACAACACCAGTGAAT GFP-RIGHT PRIMER,
aacagctatgaccatg reverse primer,
attaaccctcactaaag T3 primer,
cgaggtcgacggtatcg KS primer,
tctagaactagtggatc SK primer,
aatacgactcactatag T7 primer,
gtaaaacgacggccagt M13 primer,
gttttcccagtcacgac -40 primer,
atttaggtgacactatag Sp6
CTAGTTATTGCTCAGCGG MMUM050726a,
CAGTCGACGAATTCTTTTGTTTAACTTTAAGAAGGAGA MMUM050726b,
CAGTCGACGAATTCCAGGAAACAGAATGGTTGCAT MMUM050726c,
CCTCGAGTGTAAAACGACGGCCAGTG MMUM050726d,
AGAATTCGGGCTTTGTTAGCAG MMUM051214a,
ATACTGCAGTACGTCGACGATAATGGCCTGCTTCTCG MMUM051214b,
GATCGCATGCAGGCCATCCTGACGGATGGCCTTTTTGTGAGCGGATAACAAGTCGAC MMUM060314a,
GATCGTCGACTTGTTATCCGCTCACAAAAAGGCCATCCGTCAGGATGGCCTGCATGC MMUM060314b,
GATCGCATGCGAATTGTGAGCGGATAACAATTGTCGAC MMUM060314c,
GATCGTCGACAATTGTTATCCGCTCACAATTCGCATGC MMUM060314d,
GTTGATCGCGCTTTGATACA MMUM060314e,
ACAAGACTGGACCATCACCA MMUM060314f,
TGGGACAACTCCAGTGAAAA MMUM060314g,
More Primers
MMUM050726a Cro-Xho pET-lacZ primer 18mer 5'-CTAGTTATTGCTCAGCGG-3'
MMUM050726b Sal-R1-Cro-Xho pET-SD primer Sal1-EcoR1 24mer Tm 53.91 +14bp = 38mer 5'-CAGTCGACGAATTCTTTTGTTTAACTTTAAGAAGGAGA-3'
MMUM050726c Sal-R1-Cro-Xho Cro SD primer + Sal1-EcoR1 21 + 14 = 35 5'-CAGTCGACGAATTCCAGGAAACAGAATGGTTGCAT-3'
MMUM050726d Cro-Xho Cro Cterm primer Xho 19 + 5 + 1 = 26 5'-CCTCGAGTGTAAAACGACGGCCAGTG-3'
MMUM050213-1 ccaGGCATCACGGCAATATAC P1-21, Wanner Att lambda (AttB) left
MMUM050213-2 ggcTCTGGTCTGGTAGCAATG P4-21, Wanner Att lambda (AttB) right
MMUM050213-3 aacACTTAACGGCTGACATGG P2-21, Wanner Att Crim Plasmid right
MMUM050213-4 aACGAGTATCGAGATGGCAca P3-21, Wanner Att Crim Plasmid left
MMUM050213-5 gatccgcgtgTTGACTatttTACCTCtgg T1-29, PR-lacO BamH1-XhoI
MMUM050213-6 gatccgcgtgTTGACTatttTAtCaCtgg T1c, PR-lacO BamH1-XhoI
MMUM050213-7 gatccgcgtgTTGACTatttTcCCTgtgg T1t, PR-lacO BamH1-XhoI
MMUM050213-8 CAATTcaaccatTATCACcgccaGAGGTAaaatagtcaacacgcg B1-45, PR-lacO BamH1-XhoI
MMUM050213-9 CAATTcaaccatTATCACcgccaGtGaTAaaatagtcaacacgcg B1t, PR-lacO BamH1-XhoI
MMUM050213-10 CAATTcaaccatTATCACcgccacAGGgAaaatagtcaacacgcg B1c, PR-lacO BamH1-XhoI
MMUM050213-11 cggtGATAATggttgaattgtgagcggataacaattcccc T2-40, PR-lacO BamH1-XhoI
MMUM050213-12 tcgaggggAATTGTTATCCGCTCA B2-24, PR-lacO BamH1-XhoI
MMUM060314a, gatcGCATGCaggccatcctgacggatggccttttTGTGAGCgGaTaACAAGTCGAC, Sph-rrnB_T2-lacO-SalI 14+54=68 -
MMUM060314b, gatcGTCGACTTGTtAtCcGCTCACAaaaaggccatccgtcaggatggcctGCATGC, SalI-lacO-rrnB_T2-SphI 38+54 = 92
MMUM060314c, gatcGCATGCgaatTGTGAGCgGaTaACAAttGTCGAC, Sph-lacO-SalI O-O=16+54=70bp
MMUM060314d, gatcGTCGACaaTTGTtAtCcGCTCACAattcGCATGC, SalI-lacO-SphI 17+54=71
MMUM060314e, GTTGATCGCGCTTTGATACA, marR -3908; -3686.
MMUM060314f, ACAAGACTGGACCATCACCA, jqr-4640.
MMUM060314g, TGGGACAACTCCAGTGAAAA, jbxyhR: -4096.