Sequence Extractor - Results | |||||||||||||||||||||||||||||||||||||||||||||
Template: pUCroRS sequence EcoRI to Sma I fragment 500bp of pAP119 pasted into pUC18 EcoRI(455) to HincII. Length: 3098 bp.
RestStart
PCRStart
AatII
gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcact
^10 ^20 ^30 ^40 ^50 ^60 ^70
ctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtga
--------------------------------------------------------------------------------
tttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaata
^90 ^100 ^110 ^120 ^130 ^140 ^150
aaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttat
--------------------------------------------------------------------------------
SspI
accctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt
^170 ^180 ^190 ^200 ^210 ^220 ^230
tgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaa
--------------------------------------------------------------------------------
ttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgca
^250 ^260 ^270 ^280 ^290 ^300 ^310
aacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgt
--------------------------------------------------------------------------------
cgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgat
^330 ^340 ^350 ^360 ^370 ^380 ^390
gctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttacta
--------------------------------------------------------------------------------
DraI
gagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatac
^410 ^420 ^430 ^440 ^450 ^460 ^470
ctcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatg
--------------------------------------------------------------------------------
ScaI
actattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaatta
^490 ^500 ^510 ^520 ^530 ^540 ^550
tgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaat
--------------------------------------------------------------------------------
PvuI
tgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaac
^570 ^580 ^590 ^600 ^610 ^620 ^630
acgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattg
--------------------------------------------------------------------------------
cgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg
^650 ^660 ^670 ^680 ^690 ^700 ^710
gcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgc
--------------------------------------------------------------------------------
acgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagct
^730 ^740 ^750 ^760 ^770 ^780 ^790
tgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcga
--------------------------------------------------------------------------------
VspI
tcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctg
^810 ^820 ^830 ^840 ^850 ^860 ^870
agggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgac
--------------------------------------------------------------------------------
gtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccct
^890 ^900 ^910 ^920 ^930 ^940 ^950
caaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcggga
--------------------------------------------------------------------------------
cccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcc
^970 ^980 ^990 ^1000 ^1010 ^1020 ^1030
gggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacgg
--------------------------------------------------------------------------------
DraI
tcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatt
^1050 ^1060 ^1070 ^1080 ^1090 ^1100 ^1110
agtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaa
--------------------------------------------------------------------------------
DraI
taaaaggttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatct
^1130 ^1140 ^1150 ^1160 ^1170 ^1180 ^1190
attttccaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattaga
--------------------------------------------------------------------------------
gctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaag
^1210 ^1220 ^1230 ^1240 ^1250 ^1260 ^1270
cgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttc
--------------------------------------------------------------------------------
gtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactc
^1290 ^1300 ^1310 ^1320 ^1330 ^1340 ^1350
cattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgag
--------------------------------------------------------------------------------
tgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccg
^1370 ^1380 ^1390 ^1400 ^1410 ^1420 ^1430
acatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggc
--------------------------------------------------------------------------------
ggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttg
^1450 ^1460 ^1470 ^1480 ^1490 ^1500 ^1510
ccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaac
--------------------------------------------------------------------------------
gagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggc
^1530 ^1540 ^1550 ^1560 ^1570 ^1580 ^1590
ctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccg
--------------------------------------------------------------------------------
ggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttt
^1610 ^1620 ^1630 ^1640 ^1650 ^1660 ^1670
cctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaa
--------------------------------------------------------------------------------
atagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaa
^1690 ^1700 ^1710 ^1720 ^1730 ^1740 ^1750
tatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggataccttt
--------------------------------------------------------------------------------
aacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccc
^1770 ^1780 ^1790 ^1800 ^1810 ^1820 ^1830
ttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaatagggg
--------------------------------------------------------------------------------
tgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagt
^1850 ^1860 ^1870 ^1880 ^1890 ^1900 ^1910
actaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctca
--------------------------------------------------------------------------------
VspI PvuII
cagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctgg
^1930 ^1940 ^1950 ^1960 ^1970 ^1980 ^1990
gtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgacc
--------------------------------------------------------------------------------
VspI
cacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
^2010 ^2020 ^2030 ^2040 ^2050 ^2060 ^2070
gtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggt
--------------------------------------------------------------------------------
5' aacagctatg
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatg
^2090 ^2100 ^2110 ^2120 ^2130 ^2140 ^2150
ccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatac
--------------------------------------------------------------------------------
accatg 3' reverse primer
EcoRI
accatgattacGAATTCtcatgtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtcaggcagccat
^2170 ^2180 ^2190 ^2200 ^2210 ^2220 ^2230
tggtactaatgCTTAAGagtacaaactgtcgaatagtagctgacgtgccacgtggttacgaagaccgcagtccgtcggta
--------------------------------------------------------------------------------
cggaagctgtggattggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataa
^2250 ^2260 ^2270 ^2280 ^2290 ^2300 ^2310
gccttcgacacctaaccgacacgtccagcatttagtgacgtattaagcacagcgagttccgcgtgagggcaagacctatt
--------------------------------------------------------------------------------
VspI
SspI HincII
tgttttttgcgccgacatataaacggttctggcaaatattctgaaatgagctgTTGACAattaatcatccggctcgTATA
^2330 ^2340 ^2350 ^2360 ^2370 ^2380 ^2390
acaaaaaacgcggctgtatatttgccaagaccgtttataagactttactcgacAACTGTtaattagtaggccgagcATAT
--------------------------------------------------------------------------------
NcoI
M E Q
ATgtgtggAATTGTgAgCgGaTaACAATTtcacacaggaaacagaatggttgcatgtactaaggaggttccAtgGaaCaa
^2410 ^2420 ^2430 ^2440 ^2450 ^2460 ^2470
TAcacaccTTAACAcTcGcCtAtTGTTAAagtgtgtcctttgtcttaccaacgtacatgattcctccaaggTacCttGtt
--------------------------------------------------------------------------------
DraI BglII
R I T L K D Y A M R F G Q T K T A K D L G V Y Q S A I
CgcAtaActTtaAaaGatTatGcaAtgCgcTttGgcCaaAccAagAcaGctAaaGatCtcGgcGtgTatCaaAgcGcgAt
^2490 ^2500 ^2510 ^2520 ^2530 ^2540 ^2550
GcgTatTgaAatTttCtaAtaCgtTacGcgAaaCcgGttTggTtcTgtCgaTttCtaGagCcgCacAtaGttTcgCgcTa
--------------------------------------------------------------------------------
NaeI SspI BamHI
N K A I H A G R K I F L T I N A D G S V Y A E E V K P
cAacAagGccAtcCatGccGgcCgaAaaAtaTttTtgActAtaAacGctGatGgaTccGtaTacGcgGaaGagGtaAagC
^2570 ^2580 ^2590 ^2600 ^2610 ^2620 ^2630
gTtgTtcCggTagGtaCggCcgGctTttTatAaaAacTgaTatTtgCgaCtaCctAggCatAtgCgcCttCtcCatTtcG
--------------------------------------------------------------------------------
3' tgaccggcagcaaaatg
SphI
AvaI PstI HindIII
F P S N K K T T A Z
ccTtcCcgAgtAacAaaAaaAcaAcaGcataaataacccgacCTGCAGgcatgcAAGCTTggcactggccgtcgttttac
^2650 ^2660 ^2670 ^2680 ^2690 ^2700 ^2710
ggAagGgcTcaTtgTttTttTgtTgtCgtatttattgggctgGACGTCcgtacgTTCGAAccgtgaccggcagcaaaatg
--------------------------------------------------------------------------------
3' cagcactgacccttttg 5' -40 primer
5' M13 primer
PvuII
aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat
^2730 ^2740 ^2750 ^2760 ^2770 ^2780 ^2790
ttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcatta
--------------------------------------------------------------------------------
PvuI NarI
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttct
^2810 ^2820 ^2830 ^2840 ^2850 ^2860 ^2870
tcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaaga
--------------------------------------------------------------------------------
NdeI
ccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagcc
^2890 ^2900 ^2910 ^2920 ^2930 ^2940 ^2950
ggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcgg
--------------------------------------------------------------------------------
agccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtg
^2970 ^2980 ^2990 ^3000 ^3010 ^3020 ^3030
tcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacac
--------------------------------------------------------------------------------
RestEnd
PCREnd
accgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga
^3050 ^3060 ^3070 ^3080 ^3090
tggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgct
----------------------------------------------------------
Sequence Extractor copyright © 2001, 2003 Paul Stothard
email: stothard@ualberta.ca |
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