Sequence Extractor - Results
Template: pUCroRS sequence EcoRI to Sma I fragment 500bp of pAP119 pasted into pUC18 EcoRI(455) to HincII.
Length: 3098 bp.

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RestStart PCRStart AatII gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcact ^10 ^20 ^30 ^40 ^50 ^60 ^70 ctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtga -------------------------------------------------------------------------------- tttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaata ^90 ^100 ^110 ^120 ^130 ^140 ^150 aaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttat -------------------------------------------------------------------------------- SspI accctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt ^170 ^180 ^190 ^200 ^210 ^220 ^230 tgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaa -------------------------------------------------------------------------------- ttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgca ^250 ^260 ^270 ^280 ^290 ^300 ^310 aacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgt -------------------------------------------------------------------------------- cgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgat ^330 ^340 ^350 ^360 ^370 ^380 ^390 gctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttacta -------------------------------------------------------------------------------- DraI gagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatac ^410 ^420 ^430 ^440 ^450 ^460 ^470 ctcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatg -------------------------------------------------------------------------------- ScaI actattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaatta ^490 ^500 ^510 ^520 ^530 ^540 ^550 tgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaat -------------------------------------------------------------------------------- PvuI tgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaac ^570 ^580 ^590 ^600 ^610 ^620 ^630 acgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattg -------------------------------------------------------------------------------- cgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg ^650 ^660 ^670 ^680 ^690 ^700 ^710 gcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgc -------------------------------------------------------------------------------- acgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagct ^730 ^740 ^750 ^760 ^770 ^780 ^790 tgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcga -------------------------------------------------------------------------------- VspI tcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctg ^810 ^820 ^830 ^840 ^850 ^860 ^870 agggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgac -------------------------------------------------------------------------------- gtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccct ^890 ^900 ^910 ^920 ^930 ^940 ^950 caaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcggga -------------------------------------------------------------------------------- cccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcc ^970 ^980 ^990 ^1000 ^1010 ^1020 ^1030 gggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacgg -------------------------------------------------------------------------------- DraI tcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatt ^1050 ^1060 ^1070 ^1080 ^1090 ^1100 ^1110 agtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaa -------------------------------------------------------------------------------- DraI taaaaggttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatct ^1130 ^1140 ^1150 ^1160 ^1170 ^1180 ^1190 attttccaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattaga -------------------------------------------------------------------------------- gctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaag ^1210 ^1220 ^1230 ^1240 ^1250 ^1260 ^1270 cgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttc -------------------------------------------------------------------------------- gtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactc ^1290 ^1300 ^1310 ^1320 ^1330 ^1340 ^1350 cattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgag -------------------------------------------------------------------------------- tgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccg ^1370 ^1380 ^1390 ^1400 ^1410 ^1420 ^1430 acatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggc -------------------------------------------------------------------------------- ggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttg ^1450 ^1460 ^1470 ^1480 ^1490 ^1500 ^1510 ccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaac -------------------------------------------------------------------------------- gagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggc ^1530 ^1540 ^1550 ^1560 ^1570 ^1580 ^1590 ctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccg -------------------------------------------------------------------------------- ggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttt ^1610 ^1620 ^1630 ^1640 ^1650 ^1660 ^1670 cctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaa -------------------------------------------------------------------------------- atagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaa ^1690 ^1700 ^1710 ^1720 ^1730 ^1740 ^1750 tatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggataccttt -------------------------------------------------------------------------------- aacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccc ^1770 ^1780 ^1790 ^1800 ^1810 ^1820 ^1830 ttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaatagggg -------------------------------------------------------------------------------- tgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagt ^1850 ^1860 ^1870 ^1880 ^1890 ^1900 ^1910 actaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctca -------------------------------------------------------------------------------- VspI PvuII cagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctgg ^1930 ^1940 ^1950 ^1960 ^1970 ^1980 ^1990 gtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgacc -------------------------------------------------------------------------------- VspI cacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca ^2010 ^2020 ^2030 ^2040 ^2050 ^2060 ^2070 gtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggt -------------------------------------------------------------------------------- 5' aacagctatg ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatg ^2090 ^2100 ^2110 ^2120 ^2130 ^2140 ^2150 ccgaaatgtgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatac -------------------------------------------------------------------------------- accatg 3' reverse primer EcoRI accatgattacGAATTCtcatgtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtcaggcagccat ^2170 ^2180 ^2190 ^2200 ^2210 ^2220 ^2230 tggtactaatgCTTAAGagtacaaactgtcgaatagtagctgacgtgccacgtggttacgaagaccgcagtccgtcggta -------------------------------------------------------------------------------- cggaagctgtggattggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataa ^2250 ^2260 ^2270 ^2280 ^2290 ^2300 ^2310 gccttcgacacctaaccgacacgtccagcatttagtgacgtattaagcacagcgagttccgcgtgagggcaagacctatt -------------------------------------------------------------------------------- VspI SspI HincII tgttttttgcgccgacatataaacggttctggcaaatattctgaaatgagctgTTGACAattaatcatccggctcgTATA ^2330 ^2340 ^2350 ^2360 ^2370 ^2380 ^2390 acaaaaaacgcggctgtatatttgccaagaccgtttataagactttactcgacAACTGTtaattagtaggccgagcATAT -------------------------------------------------------------------------------- NcoI M E Q ATgtgtggAATTGTgAgCgGaTaACAATTtcacacaggaaacagaatggttgcatgtactaaggaggttccAtgGaaCaa ^2410 ^2420 ^2430 ^2440 ^2450 ^2460 ^2470 TAcacaccTTAACAcTcGcCtAtTGTTAAagtgtgtcctttgtcttaccaacgtacatgattcctccaaggTacCttGtt -------------------------------------------------------------------------------- DraI BglII R I T L K D Y A M R F G Q T K T A K D L G V Y Q S A I CgcAtaActTtaAaaGatTatGcaAtgCgcTttGgcCaaAccAagAcaGctAaaGatCtcGgcGtgTatCaaAgcGcgAt ^2490 ^2500 ^2510 ^2520 ^2530 ^2540 ^2550 GcgTatTgaAatTttCtaAtaCgtTacGcgAaaCcgGttTggTtcTgtCgaTttCtaGagCcgCacAtaGttTcgCgcTa -------------------------------------------------------------------------------- NaeI SspI BamHI N K A I H A G R K I F L T I N A D G S V Y A E E V K P cAacAagGccAtcCatGccGgcCgaAaaAtaTttTtgActAtaAacGctGatGgaTccGtaTacGcgGaaGagGtaAagC ^2570 ^2580 ^2590 ^2600 ^2610 ^2620 ^2630 gTtgTtcCggTagGtaCggCcgGctTttTatAaaAacTgaTatTtgCgaCtaCctAggCatAtgCgcCttCtcCatTtcG -------------------------------------------------------------------------------- 3' tgaccggcagcaaaatg SphI AvaI PstI HindIII F P S N K K T T A Z ccTtcCcgAgtAacAaaAaaAcaAcaGcataaataacccgacCTGCAGgcatgcAAGCTTggcactggccgtcgttttac ^2650 ^2660 ^2670 ^2680 ^2690 ^2700 ^2710 ggAagGgcTcaTtgTttTttTgtTgtCgtatttattgggctgGACGTCcgtacgTTCGAAccgtgaccggcagcaaaatg -------------------------------------------------------------------------------- 3' cagcactgacccttttg 5' -40 primer 5' M13 primer PvuII aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat ^2730 ^2740 ^2750 ^2760 ^2770 ^2780 ^2790 ttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcatta -------------------------------------------------------------------------------- PvuI NarI agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttct ^2810 ^2820 ^2830 ^2840 ^2850 ^2860 ^2870 tcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaaga -------------------------------------------------------------------------------- NdeI ccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagcc ^2890 ^2900 ^2910 ^2920 ^2930 ^2940 ^2950 ggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcgg -------------------------------------------------------------------------------- agccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtg ^2970 ^2980 ^2990 ^3000 ^3010 ^3020 ^3030 tcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacac -------------------------------------------------------------------------------- RestEnd PCREnd accgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga ^3050 ^3060 ^3070 ^3080 ^3090 tggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgct ----------------------------------------------------------

Restriction Summary
AatII gacgt|c: 70.
AccIII t|ccgga: none.
ApaI gggcc|c: none.
AvaI c|ycgrg: 2646.
BamHI g|gatcc: 2614.
BclI t|gatca: none.
BglII a|gatct: 2535.
BssHII g|cgcgc: none.
ClaI at|cgat: none.
DraI ttt|aaa: 411, 1103, 1122, 2492.
EcoRI g|aattc: 2173.
EcoRV gat|atc: none.
HincII gty|rac: 2376.
HindIII a|agctt: 2696.
HpaI gtt|aac: none.
KpnI ggtac|c: none.
MluI a|cgcgt: none.
NaeI gcc|ggc: 2580.
NarI gg|cgcc: 2861.
NcoI c|catgg: 2471.
NdeI ca|tatg: 2913.
NheI g|ctagc: none.
NotI gc|ggccgc: none.
NruI tcg|cga: none.
PstI ctgca|g: 2688.
PvuI cgat|cg: 620, 2822.
PvuII cag|ctg: 1997, 2791.
SacI gagct|c: none.
SacII ccgc|gg: none.
SalI g|tcgac: none.
ScaI agt|act: 508.
SmaI ccc|ggg: none.
SpeI a|ctagt: none.
SphI gcatg|c: 2694.
SspI aat|att: 184, 2358, 2591.
StuI agg|cct: none.
VspI at|taat: 814, 1989, 2048, 2382.
XbaI t|ctaga: none.
XhoI c|tcgag: none.
XmaI c|ccggg: none.

Primer Summary
-40 primer gttttcccagtcacgac: -2741.
M13 primer gtaaaacgacggccagt: -2721.
reverse primer aacagctatgaccatg: 2151.
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