Sequence Extractor Copyright  2001
Template: pUCOR_Cro_lac lambda OR, lacO, Cro, lacZalpha.
Length: 2000 to 2720 of 2899 bp.

  lacI->    MboII                           HinfI        PvuII
   A  P  N  T  Q  T  A  S  P  R  A  L  A  D  S  L  M  Q  L  A  R  Q  V  S  R  L                                               
GAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG-2080
CTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGAC

E  S  G  Q  Z															    HincII
GAAAGCGGGCAGtgagcgcaacgcaattaaacgttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgTTG-2160
      |oooooo   lacO3  oooooo|          |ooooo OR3 ooooo|      |ooooo OR2 ooooo|
 CTTTCGCCCGTCactcgcgttgcgttaatttgcaatTTAGATagtggcgttccctatttATAGATtgtggcacgcacaac
 <---------------------------+1      -10        Prm         -35

-35        Pr        -10        +1----------------------------------------------->
 ACTattttacctctggcggtGATAATgtgtggAATTGTGAGCgGaTaACAATTtcacacaggaaacagaatggttgcatg-2240
        |ooooo OR1 ooooo|        |ooooo  lacO1  ooooo|
tgataaaatggagaccgccactattacacaccTTAACAcTcGcCtAtTGTTAAagtgtgtcctttgtcttaccaacgtac


RsaI        NcoI                   DraI                                       BglII
       Cro->   M  E  Q  R  I  T  L  K  D  Y  A  M  R  F  G  Q  T  K  T  A  K  D
tactaaggaggttccATGGAACAACGCATAACTTTAAAAGATTATGCAATGCGCTTTGGCCAAACCAAGACAGCTAAAGA-2360
atgattcctccaaggTACCTTGTTGCGTATTGAAATTTTCTAATACGTTACGCGAAACCGGTTTGGTTCTGTCGATTTCT

                                          NaeI                              BamHI
 L  G  V  Y  Q  S  A  I  N  K  A  I  H  A  G  R  K  I  F  L  T  I  N  A  D  G  S
TCTCGGCGTGTATCAAAGCGCGATCAACAAGGCCATCCATGCCGGCCGAAAAATATTTTTGACTATAAACGCTGATGGAT-2400
AGAGCCGCACATAGTTTCGCGCTAGTTGTTCCGGTAGGTACGGCCGGCTTTTTATAAAAACTGATATTTGCGACTACCTA

                             AvaI
  V  Y  A  E  E  V  K  P  F  P  S  N  K  K  T  T  A  Z  			       5'aac
CCGTATACGCGGAAGAGGTAAAGCCCTTCCCGAGTAACAAAAAAACAACAGCAtaaataacccgacctgcaacaggaaac-2480
GGCATATGCGCCTTCTCCATTTCGGGAAGGGCTCATTGTTTTTTTGTTGTCGTatttattgggctggacgttgtcctttg


agctatgaccatg 3'reverseprimer-->
lacZM  T  M  I  S  L  A  L  A  V  V  L  Q  R  R  D  W  E  N  P  G  V  T  Q  L  N
agctATGaccATGattagcttgGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTA-2560
tcgaTACtggTACtaatcgaacCGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAAT
<-- M13primer3'tgaccggcagcaaaatg  cagcactgacccttttg5'-40primer-<

                                PvuII
  R  L  A  A  H  P  P  F  A  S  W  R  N  S  E  E  A  R  T  D  R  P  S  Q  Q  L
ATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTG-2640
TAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAAC

                     NarI                                                 NdeI
R  S  L  N  G  E  W  R end of lacZ in pUC
CGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTG-2720
GCGTCGGACTTACCGCTTACCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATACCAC