LOCUS pMS201 4249 bp DNA circular 13-FEB-2000
DEFINITION low copy vector for cloning promoters, contains pLtet01, derived
from pZS21-luc.
KEYWORDS surette.
FEATURES Location/Qualifiers
CDS 3514..4230
/vntifkey="4"
/label=GFPmut2
/note="GFP mut 2 coding sequence"
BASE COUNT 1103 a 974 c 909 g 1263 t
BclI BglII
ctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggc
^3290 ^3300 ^3310 ^3320 ^3330 ^3340 ^3350
NarI PvuII AatII
ttcccaaccttaccagagggcgccccagctggcaattccgacgtctaagaaaccattattatcatgacattaacctataa
^3370 ^3380 ^3390 ^3400 ^3410 ^3420 ^3430
AvaI XbaI NdeI
XhoI BamHI M S K
aaataggcgtatcacgaggccctttcgtcttcacctcgaggggatcctctagatttaagaaggagatatacatATGTCTA
^3450 ^3460 ^3470 ^3480 ^3490 ^3500 ^3510
One Site
AatII gacgt|c: 3405.
BamHI g|gatcc: 3483.
BclI t|gatca: 3290.
BglII a|gatct: 3295.
BssHII g|cgcgc: 2734.
EcoRV gat|atc: 554.
HincII gty|rac: 4003.
HpaI gtt|aac: 4003.
SacI gagct|c: 2449.
SpeI a|ctagt: 1650.
SspI aat|att: 1980.
XhoI c|tcgag: 3476.
Multiple Sites
AvaI c|ycgrg: 1962, 3476.
DraI ttt|aaa: 466, 990, 1034, 3904.
NaeI gcc|ggc: 200, 2633.
NarI gg|cgcc: 2089, 3133, 3381.
NcoI c|catgg: 2699, 3680, 4204.
NdeI ca|tatg: 532, 3513, 3744.
PstI ctgca|g: 3086, 4238.
PvuII cag|ctg: 3029, 3389.
SphI gcatg|c: 2734, 4244.
XbaI t|ctaga: 1, 3489.
No Sites
AccIII t|ccgga: none.
ApaI gggcc|c: none.
ClaI at|cgat: none.
EcoRI g|aattc: none.
HindIII a|agctt: none.
KpnI ggtac|c: none.
MluI a|cgcgt: none.
NheI g|ctagc: none.
NotI gc|ggccgc: none.
NruI tcg|cga: none.
PvuI cgat|cg: none.
SacII ccgc|gg: none.
SalI g|tcgac: none.
ScaI agt|act: none.
SmaI ccc|ggg: none.
StuI agg|cct: none.
VspI at|taat: none.
XmaI c|ccggg: none.