LOCUS AY054372 5739 bp DNA circular SYN 17-OCT-2001 DEFINITION CRIM plasmid pAH125, complete sequence. ACCESSION AY054372 VERSION AY054372.1 GI:16209194 KEYWORDS . SOURCE CRIM plasmid pAH125 ORGANISM CRIM plasmid pAH125 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5739) AUTHORS Haldimann,A. and Wanner,B.L. TITLE Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria JOURNAL J. Bacteriol. 183 (21), 6384-6393 (2001) MEDLINE 21475783 PUBMED 11591683 REFERENCE 2 (bases 1 to 5739) AUTHORS Haldimann,A. and Wanner,B.L. TITLE Direct Submission JOURNAL Submitted (30-AUG-2001) Biological Sciences, Purdue University, Lilly Hall, West Lafayette, IN 47907, USA FEATURES Location/Qualifiers source 1..5739 /organism="CRIM plasmid pAH125" /mol_type="genomic DNA" /db_xref="taxon:172442" gene 149..3223 /gene="lacZ" CDS 149..3223 /gene="lacZ" /note="beta-galactosidase" /codon_start=1 /transl_table=11 /product="lacZ" /protein_id="AAL09169.1" /db_xref="GI:16209195" /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK" misc_feature 3343..3612 /note="tL3" misc_feature 3618..4013 /note="attL" misc_feature 4021..4412 /note="oriRg" gene complement(4499..5293) /gene="kan" CDS complement(4499..5293) /gene="kan" /codon_start=1 /transl_table=11 /product="aminoglycoside 3'-phosphotransferase" /protein_id="AAL09170.1" /db_xref="GI:16209196" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcccggg gatccgacaa 61 ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc agttccgctg gcggcatttt 121 aactttcttt atcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt 181 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 241 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 301 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc 361 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg 421 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa 481 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga 541 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt 601 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga 661 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg 721 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg 781 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg 841 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt 901 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg 961 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac 1021 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg 1081 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt 1141 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct 1201 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca 1261 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc 1321 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt 1381 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc 1441 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca 1501 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc 1561 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg 1621 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca 1681 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac 1741 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc 1801 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg 1861 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg 1921 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga 1981 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg 2041 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga 2101 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct 2161 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag 2221 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga 2281 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct 2341 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga 2401 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat 2461 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga 2521 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg 2581 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact 2641 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt 2701 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt 2761 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca 2821 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct 2881 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt 2941 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca 3001 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac 3061 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca 3121 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag 3181 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc 3241 atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttatcta gatccccgga 3301 attaattctc atgtttgaca gcttatcact gatcagtgaa ttaatggcga tgacgcatcc 3361 tcacgataat atccgggtag gcgcaatcac tttcgtctct actccgttac aaagcgaggc 3421 tgggtatttc ccggcctttc tgttatccga aatccactga aagcacagcg gctggctgag 3481 gagataaata ataaacgagg ggctgtatgc acaaagcatc ttctgttgag ttaagaacga 3541 gtatcgagat ggcacatagc cttgctcaaa ttggaatcag gtttgtgcca ataccagtag 3601 aaacagacga agaagctagc tttgcactgg attgcgaggc tttgtgcttc tctggagtgc 3661 gacaggtttg atgacaaaaa attagcgcaa gaagacaaaa atcaccttgc gctaatgctc 3721 tgttacaggt cactaatacc atctaagtag ttgattcata gtgactgcat atatgttgtg 3781 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 3841 tatcatttta cgtttctcgt tcagcttttt tatactaagt tggcattata aaaaagcatt 3901 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgat 3961 ttcaattttg tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggctaattc 4021 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc 4081 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa 4141 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg 4201 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct 4261 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg 4321 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac 4381 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat 4441 cttgcggccg caaaaattaa aaatgaagtt ttgacggtat cgaaccccag agtcccgctc 4501 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 4561 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 4621 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 4681 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 4741 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga 4801 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 4861 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 4921 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 4981 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 5041 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 5101 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 5161 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 5221 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 5281 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atccactaga ttattgaagc 5341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 5401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcatcgat ggcccccgat 5461 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa 5521 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct 5581 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg 5641 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac 5701 ggatggcctt tttgcgtggc cagtgccaag cttgcatgc //