Hybrid lambda/lac promoters that are repressible by lac repressor and Cro.

Oligos ordered from IDT 1/20/05
Notes:
 Sph lambda PR lacO Xba btm
Can be used to replace T7 promoter in pET vectors and directly in pAH125
The HincII site in the PR promoter can be used as a diagnostic.
  HincII gty|rac: 9
          [ -35]  lambda PR      [ -10]
     cgcgtgTTGACTattttacctctggcggtGATAATggttgaattgtgagcggataacaattcccct
 gtacgcgcacaactgataaaATGGAGaccgcCACTATtaccaacTTAACACTCGCCTATTGTTAAggggagatc
gcatg|c SphI       [---lambdaoR1---]       [-------lac O-------]XbaI t|ctaga


For Uri Alon vectors (GFP and Lux)

g|gatcc   [ -35]  lambda PR      [ -10]                               c|tcgag
  gatccgcgtgTTGACTattttacctc|tggcggtGATAATggttgaattgtgagcggataacaattcccc
      gcgcacaactgataaaATGGAGaccgcCACTATtaccaacTTAACAC|TCGCCTATTGTTAAggggagct
cctag|g BamHI                                                    XhoI gagct|c

 |------T1 - 29mer------------| |-----------T2 - 40mer-------------------|
g|gatcc     [ -35]   lambda PR      [ -10]                              c|tcgag
5'gatccgcgtgTTGACTatttTACCTCtgg cggtGATAATggttgaattg-tgagcggataacaattcccc     3'
3'    gcgcacaactgataaaATGGAGacc-gcCACTATtaccaacTTAAC ACTCGCCTATTGTTAAggggagct 5'
cctag|g BamHI                                                      XhoI gagct|c
     |----------------B1-45mer---------------------| |-------B2-24mer--------|
                     OR1
gatccgcgtgTTGACTatttTACCTCtgg
    gcgcacaactgataaaATGGAGacc-gcCACTATtaccaacTTAAC

                    consensus
gatccgcgtgTTGACTatttTAtCaCtgg
    gcgcacaactgataaaATaGtGacc-gcCACTATtaccaacTTAAC

                    trashed
gatccgcgtgTTGACTatttTcCCTgtgg
    gcgcacaactgataaaAgGGAcacc-gcCACTATtaccaacTTAAC

T1-29	gatccgcgtgTTGACTatttTACCTCtgg
T2-40	cggtGATAATggttgaattgtgagcggataacaattcccc

B1-45	CAATTcaaccatTATCACcgccaGAGGTAaaatagtcaacacgcg
B2-24	tcgaggggAATTGTTATCCGCTCA

T1c		gatccgcgtgTTGACTatttTAtCaCtgg
T1t		gatccgcgtgTTGACTatttTcCCTgtgg

B1t		CAATTcaaccatTATCACcgccaGtGaTAaaatagtcaacacgcg
B1c		CAATTcaaccatTATCACcgccacAGGgAaaatagtcaacacgcg

3-29mers, 3-45mers, 1-40mer, 1-24mer
3*(29 + 45) + 40 + 24 = 286 * .35 = 100.1	286 * .55 = 157.3